Manual

From TLMtracker

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TLM-tracker performs a joint analysis of simultaneously achieved bright field and fluorescence image data. This analysis allows the temporal and spatial resolution of single cell growth and fluorescence intensity. TLM-tracker requires a sequence of bright field and/or fluorescence images of selected sections of the object at distinct time points. A good practical introduction into time-lapse microscopy and sample preparation is available here.

Contents

  1. Preprocessing
  2. Segmentation
  3. Tracking
  4. Export
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