The image analysis process with TLM-Tracker is performed as a workflow of 4 steps:
- In this step images are prepared for the segmentation process.
- This step accomplishs the recognition of cells by TLM-Tracker. In addtion, cell properties like size, position and fluorescence intensity are achieved. This step requires some elaborateness and is cruical for the significance of the result. Several additional pages explain the details of this process:
- In this step cells are tracked through the time-lapse movie to build lineage tree of all cells analysed.
- In this step the analysis results are saved to a csv or xls file.